英文论文


文献类型
Journal article (JA)
题名
Mepanipyrim induces cardiotoxicity of zebrafish (Danio rerio) larvae via promoting AhR-regulated COX expression pathway
作者
Shen, Chao (1); He, Jing (1); Zhu, Kongyang (1); Zheng, Naying (1); Yu, Yue (1); He, Chengyong (1); Yang, Chunyan (1); Zuo, Zhenghong (1, 2)
作者单位
(1) State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Xiamen University, Xiamen; 361005, China (2) State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen; 361005, China
通讯作者地址
Email
ResearchID
ORCID
期刊名称
Journal of Environmental Sciences (China)
出版社
Chinese Academy of Sciences
ISSN
1001-0742
出版信息
2023-03, 125:650-661.
JCR
影响因子
ISBN
基金
会议名称
会议地点
会议开始日期
会议结束日期
关键词
Muscle; Pesticides; Polymerase chain reaction
摘要
The wide use of pesticides has seriously threatened human health and the survival of beneficial organisms. The fungicide mepanipyrim is widely used in viticulture practices. Studies of mepanipyrim-induced toxicity in organisms are still scarce, especially studies on cardiotoxicity. In this study, we aimed to investigate mepanipyrim-induced cardiotoxicity in zebrafish (Danio rerio) larvae. We found that mepanipyrim could induce cardiotoxicity by altering the heart rate and cardiomyocyte diameter of larvae. Meanwhile, RNA sequencing and RT-qPCR data indicated that mepanipyrim exposure could dramatically alter the mRNA expression of calcium signaling pathway-, cardiac muscle contraction-, and oxidative respiratory chain-related genes. Interestingly, by the CALUX cell bioassay, we found that most cytochrome c oxidase (COX) family genes exhibited potential AhR-regulated activity, suggesting that mepanipyrim induced cardiotoxicity via a novel AhR-regulated manner in larvae. Additionally, the AhR antagonist CH223191 could effectively prevent mepanipyrim-induced cardiotoxicity in zebrafish larvae. In conclusion, the AhR agonist mepanipyrim could induce cardiotoxicity in a novel unreported AhR-regulated manner, which could specifically affect the expression of COX family genes involved in the mitochondrial oxidative respiratory chain. Our data will help explain the toxic effects of mepanipyrim on organisms and provide new insight into the AhR agonistic activity pesticide-induced cardiotoxicity. ? 2022
一级学科
WOS入藏号
EI收录号
20221411917860
DOI
10.1016/j.jes.2022.01.012
ESI
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