英文论文
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文献类型
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Journal article (JA)
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题名
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Interaction of chromium(III) or chromium(VI) with catalase and its effect on the structure and function of catalase: An in vitro study
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作者
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Chen, Linfeng(1); Zhang, Jing(2); Zhu, Yaxian(3); Zhang, Yong(1)
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作者单位
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(1) State Key Laboratory of Marine Environmental Sciences of China (Xiamen University), College of Environment and Ecology, Xiamen University, Xiamen; 361102, China; (2) Key Laboratory of Estuarine Ecological Security and Environmental Health, Tan Kah Kee College, Xiamen University, Zhangzhou; 363105, China; (3) Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen; 361005, China
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通讯作者地址
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Email
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ResearchID
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ORCID
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期刊名称
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Food Chemistry
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出版社
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Elsevier Ltd
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ISSN
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0308-8146
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出版信息
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2018-04-01, 244:378-385.
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JCR
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2
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影响因子
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5.399
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ISBN
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基金
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会议名称
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会议地点
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会议开始日期
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会议结束日期
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关键词
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Bins - Chemical contamination - Chromium compounds - Dichroism - Fluorescence - Health risks - Heavy metals - Molecular modeling - Spectroscopic analysis
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摘要
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Heavy metal chromium (Cr) poses a severe health risk to humans via food chain contamination. In this study, the interactions of either trivalent chromium (Cr(III)) or hexavalent chromium (Cr(VI)) with catalase (CAT) were investigated via multi-spectroscopic studies and computational simulations. The fluorescence analysis showed that Cr(III) and Cr(VI) quenched the fluorescence of CAT through a dynamic and a static quenching mechanism, respectively. The binding constant of Cr(VI) with CAT was 3.44 × 104l mol?1at 298 K. Other detailed binding characterizations of the Cr(VI)–CAT complex were also obtained using spectra analysis and molecular docking. Synchronous fluorescence, UV–vis and circular dichroism (CD) spectral studies showed that either Cr(III) or Cr(VI) induced conformational changes of CAT, but the degree of influence was different. The response of CAT activity to Cr(III) or Cr(VI) was found to be variable depending on their valence states and concentrations. ? 2017 Elsevier Ltd
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一级学科
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Chemistry, Applied; Food Science & Technology; Nutrition & Dietetics
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WOS入藏号
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WOS:000414966400050
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EI收录号
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20174204285635
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DOI
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10.1016/j.foodchem.2017.10.062
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ESI
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AGRICULTURAL
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收录于
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SCIE, EI
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