Articles


Document Type
Journal article (JA)
Title
Positive carbon dots with dual roles of nanoquencher and reference signal for the ratiometric fluorescence sensing of DNA
Author
Guo, Rubin(1); Chen, Bing(1); Li, Fenglan(1); Weng, Shaohuang(1); Zheng, Zongfu(2); Chen, Min(4); Wu, Wen(4); Lin, Xinhua(1); Yang, Chaoyong(3)
Address
(1) Department of Pharmaceutical Analysis, School of Pharmacy, Fujian Medical University, Fuzhou; 350122, China; (2) 476 Hospital of PLA, Fuzhou; 350002, China; (3) MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, The Key Laboratory of Chemical Biology of Fujian Province, State Key Laboratory of Physical Chemistry of Solid Surfaces, Collaborative Innovation Center of Chemistry for Energy Materials, Department of Chemical Engineering, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen; 361005, China; (4) Department of Orthopedic Surgery, Fujian Medical University Union Hospital, Fuzhou; 350001, China
RPAddress
Email
ResearchID
ORCID
Journal
Sensors and Actuators, B: Chemical
Publisher
Elsevier B.V.
ISSN
0925-4005
Published
2018-07-01, 264:193-201.
JCR
1
ImpactFactor
5.401
ISBN
Fund_Code
HYMC
HYDD
HYKSRQ
HYJSRQ
HYLWLB
HYJB
Keywords
DNA - Fluorescence - Fluorophores - Optical properties - Quenching
Abstract
Specific modification of carbon dots (CDs) exhibit excellent properties and are suitable for numerous applications. The exploitation of multifunctional CDs in the area of fluorescent assay is still urgent and attractive. Herein, using a simple two-step heating procedure, positive charged carbon dots (P-CDs) were prepared with the co-doping of the elements of N and S. The morphology, optical properties and stability of P-CDs was investigated. Furthermore, P-CDs show the dual functions of unprecedented quenching performance toward fluorophore-modified DNA coupling the retaining fluorescence of P-CDs self. The quenching effect and mechanism of P-CDs to fluorophore-modified DNA was investigated. The fluorescence and quenching properties of P-CDs were used to design a ratiometric fluorescence platform for DNA based on the complex of P-CDs and FAM-labeled ssDNA. The ratiometric method allows the detection of target DNA because of the different affinities of P-CDs to ssDNA and dsDNA with acceptable sensitivity and improved reproducibility, when using sequence fragment of the gene of Gardnerella vaginalis as model. This method provides a new approach to widen the future of the biological applications based on some special CDs. ? 2018 Elsevier B.V.
WOS Categories
Chemistry, Analytical; Electrochemistry; Instruments & Instrumentation
Accession Number
WOS:000428032900023
EI收录号
20181004883274
DOI
10.1016/j.snb.2018.02.175
ESI_Type
CHEMISTRY
Collection
SCIE, EI

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