Articles


Document Type
Article
Title
Isolation and identification of a novel LCI like antibacterial protein from Bacillus sp MD-5 reveals its potential application in controlling Staphylococcus aureus in food industry
Author
Wu, Yaping; Guo, Wenbin; Zhao, Jianbo; Ding, Lianshuai; Chen, Xinhua
Address
[Wu, Yaping; Guo, Wenbin; Zhao, Jianbo; Ding, Lianshuai; Chen, Xinhua] State Ocean Adm, Inst Oceanog 3, Key Lab Marine Biogenet Resources, Xiamen 361005, Fujian, Peoples R China; [Wu, Yaping; Chen, Xinhua] Fujian Agr & Forestry Univ, Fuzhou 350002, Fujian, Peoples R China; [Wu, Yaping; Guo, Wenbin; Zhao, Jianbo; Ding, Lianshuai; Chen, Xinhua] Fujian Key Lab Marine Genet Resources, Xiamen 361005, Peoples R China; [Wu, Yaping; Guo, Wenbin; Zhao, Jianbo; Ding, Lianshuai; Chen, Xinhua] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
RPAddress
Email
ResearchID
ORCID
Journal
FOOD CONTROL
Publisher
ELSEVIER SCI LTD
ISSN
0956-7135
Published
2018-07, 89:142-149.
JCR
ImpactFactor
ISBN
Fund_Code
National Key Basic Research Program of China [2015CB755903]; COMRA program [DY135-B-01]; Fujian Regional Demonstration Project of Marine Economy Innovation and Development [2014FJPT10]; Yantai Marine Economic Innovation and Development Demonstration City Industrial Chain Collaborative Innovation Project; Natural Science Foundation of Fujian Province, China [2016J05079]
HYMC
HYDD
HYKSRQ
HYJSRQ
HYLWLB
HYJB
Keywords
CONTROL LISTERIA-MONOCYTOGENES; NISIN; BACTERIOCIN; PURIFICATION; PEPTIDE; PREVALENCE; PATHOGENS; PESTICIN; SUBTILIS; AGAR
Abstract
A novel LCI like antibacterial protein produced by Bacillus sp. MD-5, which was isolated from the Arctic Ocean sediment sample during the sixth Chinese National Arctic Expedition in 2014, was purified and characterized. After saturation of Bacillus sp. MD-5 culture supernatant with ammonium sulfate and separation with ion-exchange chromatography and high performance liquid chromatography (HPLC), an antibacterial protein (MD) was purified. The MD protein was determined to be a novel LCI like antibacterial protein with molecular weight of about 7.0 kDa using liquid chromatograph-mass spectrometer (LC-MS) identification and the sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). It displayed antibacterial activity against Staphylococcus aureus at minimum inhibitory concentration (MIC) of 7.33 mu g/mL MD protein possessed high thermostability and wide range of pH tolerance, and had a certain extent of protease, surfactant and metal ion resistance. MD protein was suggested to inhibit the growth of S. aureus through inhibiting peptidoglycan biosynthesis in the view of TEM detection. These results suggested that MD may represent a novel antibacterial protein with potential application in controlling S. aureus in food industry. (C) 2018 Elsevier Ltd. All rights reserved.
WOS Categories
Food Science & Technology
Accession Number
WOS:000429630500017
EI收录号
DOI
10.1016/j.foodcont.2018.01.026
ESI_Type
AGRICULTURAL
Collection
SCIE

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