英文论文


文献类型
Journal article (JA)
题名
Rapid quantification of pathogenic Salmonella Typhimurium and total bacteria in eggs by nano-flow cytometry
作者
Mao, Cuiping; Xue, Chengfeng; Wang, Xuzheng; He, Shengbin; Wu, Lina; Yan, Xiaomei
作者单位
[Mao, Cuiping; Xue, Chengfeng; Wang, Xuzheng; He, Shengbin; Wu, Lina; Yan, Xiaomei] Xiamen Univ, Collaborat Innovat Ctr Chem Energy Mat, Key Lab Chem Biol Fujian Prov, Coll Chem & Chem Engn,Dept Chem Biol,MOE Key Lab, Xiamen 361005, Fujian, Peoples R China.
通讯作者地址
nding author), Xiamen Univ, Collaborat Innovat Ctr Chem Energy Mat, Key Lab Chem Biol Fujian Prov, Coll Chem & Chem Engn,Dept Chem Biol,MOE Key Lab, Xiamen 361005, Fujian, Peoples R China.
Email
xmyan@xmu.edu.cn
ResearchID
ORCID
期刊名称
Talanta
出版社
Elsevier B.V.
ISSN
0039-9140
出版信息
2020-09-01, 217 ():-.
JCR
2
影响因子
6.057
ISBN
基金
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [21627811, 21934004, 21521004]; National Fund for Fostering Talents of Basic Science (NFFTBS) [J1310024]
会议名称
会议地点
会议开始日期
会议结束日期
关键词
Escherichia coli; Flowmeters; Molecular biology; Nucleic acids; Salmonella
摘要
Rapid quantification of pathogenic Salmonella Typhimurium (S. Typhimurium) and total bacteria in eggs is highly desired for food safety control. However, the complexity of egg matrix presents a significant challenge for sensitive detection of bacteria. In this study, a sample pretreatment protocol, including dilution, fat dissolution, protein degradation, filtration, and washing was developed to circumvent this challenge. A laboratory-built nano-flow cytometer (nFCM) that is hundreds of fold more sensitive than the conventional flow cytometer was employed to analyze individual bacteria upon nucleic acid and immunofluorescent staining. Eggs spiked with pathogenic S. Typhimurium and harmless Escherichia coli K12 (E. coli K12) were used as the model system to optimize the sample pretreatment protocol. S. Typhimurium and total bacteria in eggs can be quantified without cultural enrichment, and the whole process of sample pretreatment, staining, and instrument analysis can be accomplished within 1.5 h. The bacterial recovery rate upon sample pretreatment, detection limit, and dynamic range for S. Typhimurium in eggs were 92%, 2 x 10(3) cells/mL, and from 2 x 10(3) to 4 x 10(8) cells/mL, respectively. The as-developed approach can specifically distinguish S. Typhimurium from other bacteria and successful application to bacterial detection in eggs freshly purchased from supermarket and spoiled eggs upon inappropriate storage was demonstrated.
一级学科
Chemistry, Analytical
WOS入藏号
WOS:000537880200022
EI收录号
20201708503579
DOI
10.1016/j.talanta.2020.121020
ESI
CHEMISTRY
收录于
SCIE, EI

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